ho 1 crispr cas9 ko plasmid Search Results


94
Santa Cruz Biotechnology crispr cas9 system
Crispr Cas9 System, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Santa Cruz Biotechnology sod2
Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and <t>SOD2</t> ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.
Sod2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Santa Cruz Biotechnology hdr plasmid
Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and <t>SOD2</t> ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.
Hdr Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Santa Cruz Biotechnology gapdh santa cruz sc420485
Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and <t>SOD2</t> ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.
Gapdh Santa Cruz Sc420485, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Santa Cruz Biotechnology ho 1 crispr cas9 ko plasmids
Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and <t>SOD2</t> ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.
Ho 1 Crispr Cas9 Ko Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ho+1+crispr+cas9+ko+plasmid/SP-A1+CRISPR%2FCas9+KO+Plasmid/pm35972707-57-9-36
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Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and SOD2 ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.

Journal: Experimental & Molecular Medicine

Article Title: Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

doi: 10.1038/emm.2014.112

Figure Lengend Snippet: Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and SOD2 ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.

Article Snippet: Predesigned small interference RNAs (siRNAs) against mouse HO-1 (sc-35555), SOD2 (sc-416560) and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Amplification, Expressing, Western Blot

siRNA knockdown of HO-1 and SOD2 enzymes does not completely block the protective effects of bucillamine. ( a ) After transfection of HEI-OC1 cell with double-stranded siRNA (HO-1, SOD2 or both), cells were pretreated with various concentrations of bucillamine for 1 h followed by the addition of 20 μ M cisplatin for 24 h. Then, cell viability was measured by MTT assays. ( b ) Cell lysates were subject to immunoblot analysis with antibodies against pro-caspase-3 and active form of caspase-3. β-actin was used as a control.

Journal: Experimental & Molecular Medicine

Article Title: Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

doi: 10.1038/emm.2014.112

Figure Lengend Snippet: siRNA knockdown of HO-1 and SOD2 enzymes does not completely block the protective effects of bucillamine. ( a ) After transfection of HEI-OC1 cell with double-stranded siRNA (HO-1, SOD2 or both), cells were pretreated with various concentrations of bucillamine for 1 h followed by the addition of 20 μ M cisplatin for 24 h. Then, cell viability was measured by MTT assays. ( b ) Cell lysates were subject to immunoblot analysis with antibodies against pro-caspase-3 and active form of caspase-3. β-actin was used as a control.

Article Snippet: Predesigned small interference RNAs (siRNAs) against mouse HO-1 (sc-35555), SOD2 (sc-416560) and control siRNA (sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Knockdown, Blocking Assay, Transfection, Western Blot, Control