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Image Search Results
Journal: Experimental & Molecular Medicine
Article Title: Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes
doi: 10.1038/emm.2014.112
Figure Lengend Snippet: Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. ( a ) Cells were pretreated with 2 m M bucillamine for 1 h followed by the addition of 20 μ M cisplatin at 12 h. Phase II antioxidant genes ( HO-1 , SOD1 and SOD2 ) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. ( b ) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.
Article Snippet: Predesigned small interference RNAs (siRNAs) against mouse HO-1 (sc-35555),
Techniques: Amplification, Expressing, Western Blot
Journal: Experimental & Molecular Medicine
Article Title: Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes
doi: 10.1038/emm.2014.112
Figure Lengend Snippet: siRNA knockdown of HO-1 and SOD2 enzymes does not completely block the protective effects of bucillamine. ( a ) After transfection of HEI-OC1 cell with double-stranded siRNA (HO-1, SOD2 or both), cells were pretreated with various concentrations of bucillamine for 1 h followed by the addition of 20 μ M cisplatin for 24 h. Then, cell viability was measured by MTT assays. ( b ) Cell lysates were subject to immunoblot analysis with antibodies against pro-caspase-3 and active form of caspase-3. β-actin was used as a control.
Article Snippet: Predesigned small interference RNAs (siRNAs) against mouse HO-1 (sc-35555),
Techniques: Knockdown, Blocking Assay, Transfection, Western Blot, Control